The Sialidase 2 Polyclonal Antibody (CAB8137) is specifically designed for research involving the detection and analysis of Sialidase 2, an enzyme involved in the removal of sialic acid residues from glycoproteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.Sialidase 2, also known as NEU2, plays a critical role in various cellular processes, including cell adhesion, migration, and signaling. Its involvement in regulating cell surface glycosylation makes it a key target for investigation in immunology, cancer research, and other fields. By binding to the Sialidase 2 protein, this polyclonal antibody allows for the accurate detection and analysis of this enzyme in a variety of cell types.
Understanding the function and activity of Sialidase 2 is essential for advancing our knowledge of glycosylation processes and their impact on various biological pathways. Research involving this enzyme has the potential to contribute valuable insights into diseases such as cancer, immune disorders, and metabolic conditions. Utilizing the Sialidase 2 Polyclonal Antibody (CAB8137) in your studies can provide valuable information for the development of targeted therapies and treatments in these areas of research.
Product Name:
NEU2 Rabbit Polyclonal Antibody
SKU:
CAB8137
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 171-380 of human NEU2 (NP_005374.2).
This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments.
Purification Method:
Affinity purification
Gene ID:
4759
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Raji cells, using NEU2 antibody (CAB8137) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
