The [KO Validated] SIRT5 Antibody (CAB5784) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific for detecting SIRT5 in human samples and has been validated for use in Western blot applications. By binding to the SIRT5 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for research in the fields of metabolism, aging, and cancer biology.SIRT5 is a member of the sirtuin family of proteins, known for their roles in regulating various cellular processes such as DNA repair, gene expression, and metabolism. SIRT5 specifically plays a crucial role in modulating protein post-translational modifications, including lysine desuccinylation and demalonylation, which can impact cellular function and metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] SIRT5 Antibody
SKU:
CAB5784
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
23408
RRID
AB_2766538
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and SIRT5 knockout (KO) 293T cells, using [KO Validated] SIRT5 Rabbit pAb (CAB5784) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates, using [KO Validated] SIRT5 Rabbit pAb (CAB5784) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
