The SIX3 Antibody (CAB17363) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in various applications, including immunohistochemistry and flow cytometry.SIX3 is known for its role in regulating gene expression during early development, specifically in the formation of the eye and central nervous system. Dysregulation of SIX3 has been linked to developmental disorders and certain cancers, making it a target of interest in the fields of developmental biology and oncology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SIX3 Antibody
SKU:
CAB17363
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HPE2, SIX3
Positive Sample:
SH-SY5Y, Rat lung
Cellular Localization:
Nucleus.
Calculated MW:
35kDa
Observed MW:
35kDa
This gene encodes a member of the sine oculis homeobox transcription factor family. The encoded protein plays a role in eye development. Mutations in this gene have been associated with holoprosencephaly type 2.
Purification Method
Affinity purification
Gene ID
6496
RRID
AB_2772233
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SIX3 Rabbit pAb (CAB17363) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
