The MCT1/SLC16A1 Antibody (CAB3013) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene. RRID AB_2764822 Gene ID 6566 Swiss Prot Synonym MCT; HHF7; MCT1; MCT1D; MCT1/SLC16A1
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MCT1/SLC16A1 Antibody
SKU:
CAB3013
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MCT, HHF7, MCT1, MCT1D, MCT1/SLC16A1
Positive Sample:
T-47D
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
54kDa
Observed MW:
43kDa
The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene. RRID AB_2764822 Gene ID 6566 Swiss Prot Synonym MCT; HHF7; MCT1; MCT1D; MCT1/SLC16A1
Purification Method:
Affinity purification
Gene ID:
6566
RRID:
AB_2764822
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from T-47D cells, using MCT1/SLC16A1 Rabbit pAb (CAB3013) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using MCT1/SLC16A1 Rabbit pAb (CAB3013) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
