The SLC27A5 Polyclonal Antibody (PAC020499) is a valuable tool for researchers studying SLC27A5, a protein involved in fatty acid metabolism and lipid homeostasis. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for use in Western blot applications. By binding to the SLC27A5 protein, this antibody enables the detection and analysis of SLC27A5 expression in various cell types, facilitating investigations in fields such as metabolism, lipid biology, and liver function.SLC27A5, also known as Very Long-Chain Acyl-Coenzyme A Synthetase (ACSVL1), plays a crucial role in the activation of very long-chain fatty acids for metabolic processes such as energy production and lipid synthesis.
Dysregulation of SLC27A5 has been associated with metabolic disorders, fatty liver disease, and other lipid-related conditions. Research into the function and regulation of SLC27A5 is essential for developing a better understanding of these diseases and identifying potential therapeutic targets.By utilizing the SLC27A5 Polyclonal Antibody (PAC020499), researchers can further elucidate the role of SLC27A5 in health and disease, leading to insights that may contribute to the development of novel treatments and interventions for metabolic disorders and liver conditions.
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO20499(SLC27A5 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 6%SDS-PAGE, Lysate: 40 µg, Lane: Mouse liver tissue, Primary antibody: PACO20499(SLC27A5 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes.
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO20499(SLC27A5 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acid, containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid, elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog.
Synonyms:
solute carrier family 27 (fatty acid, transporter), member 5
UniProt Protein Function:
SLC27A5: Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Belongs to the ATP-dependent AMP-binding enzyme family.Protein type: EC 6.2.1.7; Membrane protein, multi-pass; Endoplasmic reticulum; Ligase; Membrane protein, integral; Lipid Metabolism - primary bile acid biosynthesisChromosomal Location of Human Ortholog: 19q13.43Cellular Component: basal plasma membrane; endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; protein complexMolecular Function: ATP binding; cholate-CoA ligase activity; fatty acid transporter activity; long-chain-fatty-acid-CoA ligase activity; protein complex binding; very-long-chain-fatty-acid-CoA ligase activityBiological Process: bile acid and bile salt transport; bile acid biosynthetic process; bile acid metabolic process; ketone body biosynthetic process; long-chain fatty acid metabolic process; plasma membrane long-chain fatty acid transport; triacylglycerol mobilization; very-long-chain fatty acid metabolic process
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog. [provided by RefSeq, Jul 2008]
Bile acid-CoA ligase; BA-CoA ligase; BAL; Cholate--CoA ligase; Fatty acid transport protein 5; FATP-5; Fatty-acid-coenzyme A ligase, very long-chain 3; Solute carrier family 27 member 5; Very long-chain acyl-CoA synthetase homolog 2; VLCS-H2; VLCSH2; Very long-chain acyl-CoA synthetase-related protein; VLACS-related; VLACSR
