The SLC27A5 Polyclonal Antibody (PAC020500) is a valuable tool for researchers studying SLC27A5, a cell surface molecule known for its crucial role in fatty acid metabolism. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the SLC27A5 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for research in metabolism, lipid biology, and liver function.SLC27A5, also referred to as Very Long-Chain Acyl-CoA Synthetase, is essential for cellular uptake and activation of long-chain fatty acids, playing a vital role in lipid metabolism and energy production.
Dysregulation of SLC27A5 has been associated with various metabolic disorders, including obesity, diabetes, and fatty liver disease. By studying the function and regulation of SLC27A5, researchers can gain insights into the molecular mechanisms underlying these conditions and identify potential therapeutic targets for metabolic diseases.
Antibody Name:
SLC27A5 Antibody (PACO20500)
Antibody SKU:
PACO20500
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human SLC27A5
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 8%SDS-PAGE,Lysate: 40 μgPrimary antibody: PACO20500(SLC27A5 Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 1 minute.
Background:
The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acid, containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid, elongation or complex lipid synthesis rather than in degradation.
Synonyms:
solute carrier family 27 (fatty acid, transporter), member 5
UniProt Protein Function:
SLC27A5: Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Belongs to the ATP-dependent AMP-binding enzyme family.Protein type: EC 6.2.1.7; Membrane protein, multi-pass; Endoplasmic reticulum; Ligase; Membrane protein, integral; Lipid Metabolism - primary bile acid biosynthesisChromosomal Location of Human Ortholog: 19q13.43Cellular Component: basal plasma membrane; endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; protein complexMolecular Function: ATP binding; cholate-CoA ligase activity; fatty acid transporter activity; long-chain-fatty-acid-CoA ligase activity; protein complex binding; very-long-chain-fatty-acid-CoA ligase activityBiological Process: bile acid and bile salt transport; bile acid biosynthetic process; bile acid metabolic process; ketone body biosynthetic process; long-chain fatty acid metabolic process; plasma membrane long-chain fatty acid transport; triacylglycerol mobilization; very-long-chain fatty acid metabolic process
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog. [provided by RefSeq, Jul 2008]
Bile acid-CoA ligase; BA-CoA ligase; BAL; Cholate--CoA ligase; Fatty acid transport protein 5; FATP-5; Fatty-acid-coenzyme A ligase, very long-chain 3; Solute carrier family 27 member 5; Very long-chain acyl-CoA synthetase homolog 2; VLCS-H2; VLCSH2; Very long-chain acyl-CoA synthetase-related protein; VLACS-related; VLACSR
