The SLC6A5 Antibody (CAB11647) is a high-quality antibody developed for reliable detection and analysis of target proteins. SLC6A5 encodes a sodium- and chloride-dependent glycine transporter responsible for clearing extracellular glycine from glycinergic synapses, maintaining a high presynaptic pool of neurotransmitter. Mutations in this gene cause hyperekplexia, a neurological disorder characterized by exaggerated startle responses and neonatal apnea.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SLC6A5 Antibody
SKU:
CAB11647
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NET1, GLYT2, HKPX3, GLYT-2, SLC6A5
Positive Sample:
SH-SY5Y, U-937, U-87MG, U-251MG, Mouse brain, Mouse liver, Rat brain
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
87kDa
Observed MW:
60kDa
This gene encodes a sodium- and chloride-dependent glycine neurotransmitter transporter. This integral membrane glycoprotein is responsible for the clearance of extracellular glycine during glycine-mediated neurotransmission. This protein is found in glycinergic axons and maintains a high presynaptic pool of neurotransmitter at glycinergic synapses. Mutations in this gene cause hyperekplexia; a heterogenous neurological disorder characterized by exaggerated startle responses and neonatal apnea. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
9152
RRID
AB_2758660
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SLC6A5 Rabbit pAb (CAB11647) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
