The Smad1 Polyclonal Antibody is a valuable tool for researchers studying the Smad1 protein, an important component of the TGF-β signaling pathway. This antibody is raised in rabbits and has been validated for use in Western blot applications, allowing for the detection and analysis of Smad1 in various cell types.Smad1 is a transcription factor that plays a crucial role in the regulation of cell growth, differentiation, and development. Dysregulation of the TGF-β signaling pathway, including aberrant Smad1 activity, has been implicated in a variety of diseases such as cancer, fibrosis, and developmental disorders.
By utilizing the Smad1 Polyclonal Antibody, researchers can gain insights into the function and regulation of Smad1, paving the way for potential therapeutic interventions targeting the TGF-β signaling pathway. This antibody is suitable for use in immunology and cancer research, allowing for a deeper understanding of the molecular mechanisms underlying these diseases.
Product Name:
Smad1 Polyclonal Antibody
SKU:
CAB21734
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human Smad1 (NP_001003688.1).
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method:
Affinity purification
Gene ID:
4086
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] Smad1 Rabbit pAb (CAB21734) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
