The SMC4 Antibody (CAB13933) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot and immunohistochemistry applications.SMC4 is essential for proper chromosome segregation and DNA repair processes during cell division. Dysregulation of SMC4 has been implicated in various diseases, including cancer and genetic disorders. The SMC4 Polyclonal Antibody allows for the detection and analysis of SMC4 protein expression in different cell types, facilitating research in cell biology, oncology, and genetic studies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SMC4 Antibody
SKU:
CAB13933
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAPC, CAP-C, SMC-4, SMC4L1, SMC4
Positive Sample:
HT-29, MCF7, Raji, HeLa
Cellular Localization:
Chromosome, Cytoplasm, Nucleus.
Calculated MW:
147kDa
Observed MW:
147kDa
This gene belongs to the 'structural maintenance of chromosomes' (SMC) gene family. Members of this gene family play a role in two changes in chromosome structure during mitotic segregation of chromosomes- chromosome condensation and sister chromatid cohesion. The protein encoded by this gene is likely a subunit of the 13S condensin complex, which is involved in chromosome condensation. A pseudogene related to this gene is located on chromosome 2.
Purification Method
Affinity purification
Gene ID
10051
RRID
AB_2760785
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SMC4 Rabbit pAb (CAB13933) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3s.
