The SMCHD1 Antibody (CAB7214) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the SMCHD1 protein, this antibody allows for the detection and analysis of SMCHD1 in a variety of cell types, making it an ideal choice for studies in genetics, developmental biology, and epigenetics.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
SMCHD1 Antibody
SKU:
CAB7214
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BAMS, FSHD2, SMCHD1
Positive Sample:
HL-60, SKOV3, Jurkat, U-251MG, HepG2, Rat brain
Cellular Localization:
Chromosome.
Calculated MW:
226kDa
Observed MW:
246kDa
This gene encodes a protein which contains a hinge region domain found in members of the SMC (structural maintenance of chromosomes) protein family.
Purification Method
Affinity purification
Gene ID
23347
RRID
AB_2767763
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SMCHD1 Rabbit pAb (CAB7214) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of MCF-7 cells using SMCHD1 Rabbit pAb (CAB7214). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
