The SMG8 Polyclonal Antibody (PAC018645) is specifically designed for research involving SMG8, a protein involved in nonsense-mediated mRNA decay (NMD) pathway regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the SMG8 protein, this antibody allows for the detection and analysis of SMG8 in various cell types, making it an excellent tool for studies in molecular biology and RNA metabolism research.SMG8 is a crucial component of the NMD pathway, which serves to degrade mRNA transcripts containing premature stop codons, thereby preventing the translation of potentially harmful truncated proteins.
Dysregulation of the NMD pathway has been implicated in various genetic disorders and cancers, highlighting the importance of studying proteins like SMG8 to elucidate their roles in mRNA quality control mechanisms. By targeting SMG8, researchers can gain valuable insights into the molecular mechanisms underlying these diseases and potentially identify novel therapeutic targets for intervention.
Antibody Name:
SMG8 Antibody (PACO18645)
Antibody SKU:
PACO18645
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human SMG8
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO18645(SMG8 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO18645(SMG8 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG9 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required to mediate the recruitment of SMG1 to the ribosome:SURF complex and to suppress SMG1 kinase activity until the ribosome:SURF complex locates the exon junction complex (EJC). Acts as a regulator of kinase activity.
Synonyms:
SMG8 nonsense mediated mRNA decay factor
UniProt Protein Function:
SMG8: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG9 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required to mediate the recruitment of SMG1 to the ribosome:SURF complex and to suppress SMG1 kinase activity until the ribosome:SURF complex locates the exon junction complex (EJC). Acts as a regulator of kinase activity. Belongs to the SMG8 family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: RNA processingChromosomal Location of Human Ortholog: 17q22Cellular Component: cytosolMolecular Function: protein bindingBiological Process: mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein kinase activity
