The SMYD1 Antibody (CAB7308) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the SMYD1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it well-suited for investigations in muscle biology and related fields.SMYD1, or SET and MYND domain-containing protein 1, is essential for proper muscle differentiation and maintenance, making it a promising target for research on muscle disorders, cardiac diseases, and skeletal muscle regeneration.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SMYD1 Antibody
SKU:
CAB7308
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BOP, KMT3D, ZMYND18, ZMYND22, SMYD1
Positive Sample:
HepG2, Raji, MCF7, Mouse skeletal muscle, Rat heart, Rat skeletal muscle
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
57kDa
Observed MW:
54kDa
Predicted to enable histone-lysine N-methyltransferase activity. Involved in positive regulation of myoblast differentiation and positive regulation of myotube differentiation. Predicted to be located in cytoplasm. Predicted to be active in nucleus.
Purification Method
Affinity purification
Gene ID
150572
RRID
AB_2767848
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SMYD1 Rabbit pAb (CAB7308) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
