The SNRNP200 Antibody (CAB6063) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it an ideal choice for Western blot experiments. By binding to SNRNP200, this antibody enables researchers to accurately detect and analyze the protein in various cell types, facilitating investigations into RNA metabolism and gene expression regulation.SNRNP200 is a crucial component of the spliceosome complex, responsible for removing introns from pre-mRNA molecules during gene expression. Dysregulation of SNRNP200 has been linked to various diseases, including spinal muscular atrophy and cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SNRNP200 Antibody
SKU:
CAB6063
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BRR2, RP33, HELIC2, ASCC3L1, U5-200KD, SNRNP200
Positive Sample:
Jurkat, A-431, HepG2
Cellular Localization:
Nucleus.
Calculated MW:
245kDa
Observed MW:
244kDa
Pre-mRNA splicing is catalyzed by the spliceosome, a complex of specialized RNA and protein subunits that removes introns from a transcribed pre-mRNA segment. The spliceosome consists of small nuclear RNA proteins (snRNPs) U1, U2, U4, U5 and U6, together with approximately 80 conserved proteins. U5 snRNP contains nine specific proteins. This gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in this gene cause autosomal-dominant retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined.
Purification Method
Affinity purification
Gene ID
23020
RRID
AB_2766732
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SNRNP200 Rabbit pAb (CAB6063) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
