The SNX13 Antibody (CAB12821) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to SNX13, this antibody allows for the detection and analysis of this protein in a variety of cell types, making it ideal for investigations in cell biology and molecular biology.SNX13 is known to play a critical role in various cellular processes, including endosomal sorting and recycling pathways. Its involvement in membrane trafficking suggests its potential significance in diseases related to cellular dysfunction, such as neurodegenerative disorders and metabolic diseases.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SNX13 Antibody
SKU:
CAB12821
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RGS-PX1, SNX13
Positive Sample:
U-87MG, HeLa
Cellular Localization:
Cytoplasmic Side, Early Endosome Membrane, Peripheral Membrane Protein.
Calculated MW:
112kDa
Observed MW:
112kDa
This gene encodes a PHOX domain- and RGS domain-containing protein that belongs to the sorting nexin (SNX) family and the regulator of G protein signaling (RGS) family. The PHOX domain is a phosphoinositide binding domain, and the SNX family members are involved in intracellular trafficking. The RGS family members are regulatory molecules that act as GTPase activating proteins for G alpha subunits of heterotrimeric G proteins. The RGS domain of this protein interacts with G alpha(s), accelerates its GTP hydrolysis, and attenuates G alpha(s)-mediated signaling. Overexpression of this protein delayes lysosomal degradation of the epidermal growth factor receptor. Because of its bifunctional role, this protein may link heterotrimeric G protein signaling and vesicular trafficking.
Purification Method
Affinity purification
Gene ID
23161
RRID
AB_2759661
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SNX13 Rabbit pAb (CAB12821) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of paraffin-embedded mouse brain using SNX13 Rabbit pAb (CAB12821) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
