The SOX1 Monoclonal Antibody (CAB23447) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced through hybridoma technology and is specifically designed for high reactivity with human samples.SOX1 is a crucial regulator of neural development and plays a key role in maintaining pluripotency in embryonic stem cells. By targeting the SOX1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an invaluable tool for studies in developmental biology and regenerative medicine.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SOX1 Monoclonal Antibody
SKU:
CAB23447
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC57513
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SOX1, SRY-box 1
Positive Sample:
Neuro-2a, Mouse brain, C6
Cellular Localization:
Nucleus.
Calculated MW:
39kDa
Observed MW:
45kDa
This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. In mice, a similar protein regulates the gamma-crystallin genes and is essential for lens development.
Purification Method
Affinity purification
Gene ID
6656
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using SOX1 Rabbit mAb (CAB23447) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from C6 cells, using SOX1 Rabbit mAb (CAB23447) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
