The SOX1 Antibody (CAB3086) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the SOX1 protein, researchers can accurately detect and analyze its expression in various cell types, making it a valuable tool for studies in neurobiology, developmental biology, and regenerative medicine.SOX1 is a critical regulator of neural stem cell fate and differentiation, with implications in neurogenesis and central nervous system development. Its role in maintaining stem cell pluripotency and directing cell fate decisions makes it an attractive target for investigating neurological disorders, brain tumors, and neurodegenerative diseases.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SOX1 Antibody
SKU:
CAB3086
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SOX1, SRY-box 1
Positive Sample:
Mouse brain, Rat ovary
Cellular Localization:
Nucleus.
Calculated MW:
39kDa
Observed MW:
50kDa
This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. In mice, a similar protein regulates the gamma-crystallin genes and is essential for lens development.
Purification Method
Affinity purification
Gene ID
6656
RRID
AB_2764888
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SOX1 Rabbit pAb (CAB3086) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Mouse embryos using SOX1 Rabbit pAb (CAB3086) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
