The SOX6 Antibody (CAB7115) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.SOX6 plays a crucial role in the regulation of gene expression, particularly in the differentiation and maturation of specific cell types. By targeting the SOX6 protein, this antibody enables the detection and analysis of SOX6 expression in different cell types, making it ideal for studies in developmental biology, stem cell research, and tissue regeneration.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SOX6 Antibody
SKU:
CAB7115
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
MKGH GELQ GHER RMSS KQAT SPFA CAAD GEDA MTQD LTSR EKEE GSDQ HVAS HLPL HPIM HNKP HSEE LPTL VS
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SOXD, HSSOX6, TOLCAS, SOX6
Positive Sample:
U-87MG, HT-29, Jurkat, Mouse kidney, Mouse skeletal muscle, Rat kidney, Rat skeletal muscle
Cellular Localization:
Nucleus.
Calculated MW:
92kDa
Observed MW:
110kDa
This gene encodes a member of the D subfamily of sex determining region y-related transcription factors that are characterized by a conserved DNA-binding domain termed the high mobility group box and by their ability to bind the minor groove of DNA. The encoded protein is a transcriptional activator that is required for normal development of the central nervous system, chondrogenesis and maintenance of cardiac and skeletal muscle cells. The encoded protein interacts with other family members to cooperatively activate gene expression. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
55553
RRID
AB_2767670
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SOX6 Rabbit pAb (CAB7115) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
