The SP3 Antibody (CAB12790) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit immunization, has high specificity and sensitivity for detecting SP3 in human samples, making it an ideal choice for Western blot and immunohistochemistry applications.SP3 is a key player in cell growth, development, and differentiation, making it a crucial target for studies in cancer research and stem cell biology.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SP3 Antibody
SKU:
CAB12790
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SPR2, SP3
Positive Sample:
A-549, PC-3, Mouse liver, Mouse brain, Rat testis
Cellular Localization:
Nucleus, Pml Body.
Calculated MW:
82kDa
Observed MW:
70kDa
This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.
Purification Method
Affinity purification
Gene ID
6670
RRID
AB_2759630
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SP3 Rabbit pAb (CAB12790) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using SP3 Rabbit pAb (CAB12790) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
