The SP4 Antibody (CAB16431) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding specifically to the SP4 protein, researchers can effectively detect and analyze SP4 levels in various cell types, making it an ideal choice for studies in molecular biology and genetics.SP4 plays a crucial role in the transcriptional regulation of genes involved in cell proliferation, differentiation, and development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SP4 Antibody
SKU:
CAB16431
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HF1B, SPR-1, SP4
Positive Sample:
Mouse thymus, Mouse spleen, Mouse pancreas, Rat thymus
Cellular Localization:
Nucleus.
Calculated MW:
82kDa
Observed MW:
82kDa
The protein encoded by this gene is a transcription factor that can bind to the GC promoter region of a variety of genes, including those of the photoreceptor signal transduction system. The encoded protein binds to the same sites in promoter CpG islands as does the transcription factor SP1, although its expression is much more restricted compared to that of SP1. This gene may be involved in bipolar disorder and schizophrenia.
Purification Method
Affinity purification
Gene ID
6671
RRID
AB_2772358
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SP4 Rabbit pAb (CAB16431) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
