The SPDL1 Antibody (PAC038966) is a high-quality polyclonal antibody designed for research involving SPDL1, a key protein involved in cell division and spindle formation. This antibody is raised in rabbits and is highly specific and sensitive for detecting SPDL1 in human samples. Validated for use in Western blot applications, this antibody allows for precise detection and analysis of SPDL1 protein levels in different cell types.SPDL1, also known as spindle apparatus coiled-coil protein 1, is a crucial regulator of mitotic spindle assembly and chromosome segregation during cell division. Its role in ensuring proper cell division makes it a valuable target for research in cancer biology and developmental biology.
Understanding the function and expression of SPDL1 can provide important insights into cell division mechanisms and potential therapeutic targets for cancer treatment.With its high reactivity and specificity, the SPDL1 Antibody (PAC038966) is an essential tool for researchers studying cell division, spindle formation, and related processes in various biological contexts. Its reliable performance in Western blot applications makes it a versatile option for immunology and cancer research studies.
Western blot. All lanes: SPDL1 antibody at 4µg/ml. Lane 1: Hela whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: A549 whole cell lysate. Lane 5: K562 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 71, 59, 62 kDa. Observed band size: 71 kDa.
Immunohistochemistry of paraffin-embedded human testis tissue using PACO38966 at dilution of 1:100.
Background:
Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
Synonyms:
Protein Spindly (hSpindly) (Arsenite-related gene 1 protein) (Coiled-coil domain-containing protein 99) (Rhabdomyosarcoma antigen MU-RMS-40.4A) (Spindle apparatus coiled-coil domain-containing protein 1), SPDL1, CCDC99
UniProt Protein Function:
CCDC99: Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Belongs to the Spindly family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 5q35.1Cellular Component: cytosol; nucleus; outer kinetochore of condensed chromosome; spindle poleMolecular Function: enzyme binding; kinetochore binding; protein bindingBiological Process: establishment of mitotic spindle orientation; mitotic metaphase plate congression; sister chromatid cohesion
UniProt Protein Details:
NCBI Summary:
This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling. [provided by RefSeq, Jul 2016]
