The SPDYA Antibody (CAB2153) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is validated for use in various applications including Western blot, immunoprecipitation, and immunofluorescence. It binds specifically to the Protein A tag, allowing for efficient and accurate detection and analysis of tagged proteins in different experimental settings.The Protein A tag is widely used in biotechnology and molecular biology for purification and detection of recombinant proteins.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SPDYA Antibody
SKU:
CAB2153
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SPY1, SPDY1, RINGO3, RINGOA, SPDYA
Positive Sample:
A-549, Mouse ovary, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
36kDa
Observed MW:
48kDa
Enables protein kinase activator activity and protein kinase binding activity. Involved in several processes, including G1/S transition of mitotic cell cycle; positive regulation of cell population proliferation; and positive regulation of protein kinase activity. Located in nucleoplasm.
Purification Method
Affinity purification
Gene ID
245711
RRID
AB_2764172
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SPDYA Rabbit pAb (CAB2153) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
