The SPRR1A Antibody (CAB17535) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it a valuable tool for researchers studying skin biology and related disorders.SPRR1A, also known as Small Proline-Rich Protein 1A, is involved in the formation of the cornified cell envelope, a crucial process in skin barrier function. Abnormal expression of SPRR1A has been implicated in various skin conditions, making it a target of interest in dermatological research.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SPRR1A Antibody
SKU:
CAB17535
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SPRK, SPRR1A
Positive Sample:
Mouse stomach
Cellular Localization:
Cytosol.
Calculated MW:
10kDa
Observed MW:
12kDa
A structural constituent of skin epidermis. Involved in keratinocyte differentiation and peptide cross-linking. Located in cornified envelope.
Purification Method
Affinity purification
Gene ID
6698
RRID
AB_2772376
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse stomach, using SPRR1A Rabbit pAb (CAB17535) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of paraffin-embedded mouse skin using SPRR1A Rabbit pAb (CAB17535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
