The SPRTN Antibody (CAB11663) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting SPRTN in human samples, particularly in Western blot applications. By targeting the SPRTN protein, researchers can explore its role in DNA repair mechanisms and its potential implications in cancer research and genetic diseases.SPRTN, also known as Spartan, is a key player in the DNA damage response pathway, playing a crucial role in maintaining genomic integrity and preventing the accumulation of DNA damage.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SPRTN Antibody
SKU:
CAB11663
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DVC1, PRO4323, spartan, C1orf124, SPRTN
Positive Sample:
A-549
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
55kDa
Observed MW:
60kDa
The protein encoded by this gene may play a role in DNA repair during replication of damaged DNA. This protein recruits valosin containing protein (p97) to stalled DNA replication forks where it may prevent excessive translesional DNA synthesis and limit the number of DNA-damage induced mutations. It may also be involved in replication-related G2/M-checkpoint regulation. Deficiency of a similar protein in mouse causes chromosomal instability and progeroid phenotypes. Mutations in this gene have been associated with Ruijs-Aalfs syndrome (RJALS). Alternatively spliced transcript variants have been identified.
Purification Method
Affinity purification
Gene ID
83932
RRID
AB_2758671
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-549 cells, using SPRTN Rabbit pAb (CAB11663) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
