SRB Cytotoxicity Assay (CV0009)

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ELISA Kit Technical ManualMSDS

SRB Cytotoxicity Assay

Sulforhodamine B (SRB) Cytotoxicity Assay is a sensitive, reproducible and easy-to-use assay based on the ability of SRB to bind to protein components of cells that have been fixed to tissue culture plates.

SRB is a bright-pink aminoxanthene dye with two sulfonic groups that bind to basic aminoacid residues under mild acidic conditions and dissociate under basic conditions.

As the binding of SRB is stoichiometric, the amount of dye extracted from stained cells is directly proportional to the cell mass. The fixed dye is solubilized and is measured photometrically at OD 540 nm with a reference filter of 690 nm. The OD values correlate with total protein content and therefore with cell number.

Applications, Shipping & Safety

Overview Description


  • Detection of cell toxicity, death, viability or proliferation.
  • High throughput screening.

Shipping & Storage:

  • Shipped in: Ambient Temperature.
  • Storage: Room Temperature.
  • Recommendations: Protect the product from light.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.


  • Precautions: Some components of this kit are potentially carcinogenic or corrosive; it is advisable to work in a hood and to wear glasses, gloves and a mask.
  • This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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