The SRF Antibody (CAB16718) is a high-quality antibody developed for reliable detection and analysis of target proteins. SRF is a transcription factor that plays a crucial role in the regulation of genes involved in cell growth, differentiation, and tissue remodeling. This antibody, produced in rabbits, demonstrates high specificity and sensitivity in detecting SRF in human samples, making it ideal for Western blot applications.By targeting SRF, researchers can gain insights into its function and signaling pathways, particularly in the context of cancer, cardiovascular diseases, and neurological disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SRF Antibody
SKU:
CAB16718
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MCM1, SRF
Positive Sample:
Mouse liver, Mouse lung
Cellular Localization:
Nucleus.
Calculated MW:
52kDa
Observed MW:
68kDa
This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6722
RRID
AB_2772383
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SRF Rabbit pAb (CAB16718) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
