The STAG2 Polyclonal Antibody (PACO17043) is a valuable tool for researchers studying the STAG2 protein, an essential component of the cohesin complex involved in chromosome segregation and DNA repair. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By targeting the STAG2 protein, researchers can accurately detect and analyze its expression in various cell types, providing insights into its role in cell division and genomic stability.
STAG2 is a key player in maintaining chromosomal integrity and proper cell division, making it a crucial focus in cancer research and genetic disorders. Dysregulation of STAG2 has been linked to various cancers and developmental abnormalities, highlighting its importance in understanding disease mechanisms. By utilizing the STAG2 Polyclonal Antibody, researchers can further explore the functions of STAG2 and potentially identify novel therapeutic targets for cancer treatment and genetic diseases.
Antibody Name:
STAG2 Antibody (PACO17043)
Antibody SKU:
PACO17043
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:5000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human STAG2
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Jurkat cells, K562 cells, hela cells, Primary antibody: PACO17043(STAG2 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds.
Background:
The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Synonyms:
stromal antigen 2
UniProt Protein Function:
STAG2: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3. Belongs to the SCC3 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Transcription, coactivator/corepressor
Biological Process: negative regulation of DNA endoreduplication; protein sumoylation; sister chromatid cohesion
NCBI Summary:
The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
