The STAG3 Polyclonal Antibody (PACO56366) is a valuable tool for researchers studying the STAG3 protein, a key player in meiosis and chromosomal cohesion. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the STAG3 protein, this antibody facilitates the detection and analysis of STAG3 in various cell types, making it an essential component of studies in genetics and reproductive biology.STAG3 is essential for proper chromosome segregation during meiosis, ensuring the accurate distribution of genetic material to daughter cells.
Dysregulation of STAG3 has been linked to infertility and chromosomal abnormalities, highlighting its importance in reproductive health. Research on STAG3 has implications for fertility treatments, genetic counseling, and understanding the mechanisms underlying genetic disorders. The STAG3 Polyclonal Antibody provides researchers with a reliable tool to investigate the role of STAG3 in meiosis and chromosomal dynamics, shedding light on fundamental processes in cell division.
Antibody Name:
STAG3 Antibody (PACO56366)
Antibody SKU:
PACO56366
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human Cohesin subunit SA-3 protein (1056-1212AA)
IHC image of PACO56366 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO56366 diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I.
STAG3: Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis- specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I. Belongs to the SCC3 family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 7q22.1Cellular Component: chromosome, pericentric region; extracellular space; nucleus; synaptonemal complexBiological Process: synaptonemal complex assemblyDisease: Premature Ovarian Failure 8
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is expressed in the nucleus and is a subunit of the cohesin complex which regulates the cohesion of sister chromatids during cell division. A mutation in this gene is associated with premature ovarian failure. Alternate splicing results in multiple transcript variants encoding distinct isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Apr 2014]
