The STAMBP Antibody (CAB7065) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced by immunizing rabbits and is highly reactive with human samples, making it suitable for a variety of experimental applications.STAMBP is a critical regulator of endosomal sorting and recycling, playing a key role in maintaining cellular homeostasis and proper functioning of the endocytic pathway.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
STAMBP Antibody
SKU:
CAB7065
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AMSH, MICCAP, STAMBP
Positive Sample:
MCF7, SW480, HepG2, HeLa, 293T, Mouse brain, Rat lung, Rat liver
Cellular Localization:
Cytoplasm, Early Endosome, Membrane, Nucleus, Peripheral Membrane Protein.
Calculated MW:
48kDa
Observed MW:
48kDa
Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for induction of MYC and cell growth. The protein encoded by this gene binds to the SH3 domain of the signal-transducing adaptor molecule, and plays a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Purification Method
Affinity purification
Gene ID
10617
RRID
AB_2767620
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using STAMBP Rabbit pAb (CAB7065) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using STAMBP Rabbit pAb (CAB7065) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of U2OS cells using STAMBP Rabbit pAb (CAB7065). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
