The STARD3NL Antibody (CAB16579) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to STARD3NL, it enables the detection and analysis of this protein in various cell types, making it ideal for studies in cellular biology and lipid metabolism research.STARD3NL is a member of the STARD protein family and is known to play a crucial role in the intracellular trafficking of cholesterol and other lipids.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
STARD3NL Antibody
SKU:
CAB16579
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
RLLI VQDA SERA ALIP GGLS DGQF YSPP ESEA GSEE AEEK QDSE KPLL EL
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MENTHO, STARD3NL
Positive Sample:
HeLa, 293T, MCF7, Mouse testis
Cellular Localization:
Late Endosome Membrane, Multi-Pass Membrane Protein.
Calculated MW:
27kDa
Observed MW:
26kDa
This gene encodes a late-endosomal protein that contains a conserved MENTAL (MLN64 N-terminal) domain. The encoded protein binds cholesterol molecules and may play a role in endosomal cholesterol transport through interactions with metastatic lymph node protein 64 (MLN64).
Purification Method
Affinity purification
Gene ID
83930
RRID
AB_2772421
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using STARD3NL Rabbit pAb (CAB16579) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using STARD3NL Rabbit pAb (CAB16579) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
