The STAT6 Polyclonal Antibody (CAB24832) is a high-quality antibody developed for reliable detection and analysis of target proteins. STAT6 is a member of the STAT family of transcription factors that, upon phosphorylation by receptor-associated kinases in response to cytokines, forms dimers that translocate to the nucleus to activate transcription. It plays a central role in IL-4-mediated biological responses including induction of the anti-apoptotic factor BCL-XL, differentiation of Th2 cells, and immunoglobulin class switching.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
STAT6 Polyclonal Antibody
SKU:
CAB24832
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
STAT6, D12S1644, IL-4-STAT, STAT6B, STAT6C, signal transducer and activator of transcription 6
Positive Sample:
MCF7, U-251 MG
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
74kDa/81kDa/94kDa
Observed MW:
94kDa
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
6778
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using STAT6 Rabbit pAb (CAB24832) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
