The STX17 Antibody (CAB17174) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications. By targeting the STX17 protein, this antibody allows for precise detection and analysis in various cell types, making it ideal for investigations in cell biology and molecular biology research.STX17 is essential for the regulation of vesicle transport and fusion events within cells, making it a crucial player in processes such as autophagy and exocytosis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
STX17 Antibody
SKU:
CAB17174
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
STX17
Positive Sample:
Mouse heart
Cellular Localization:
Autophagosome, Copii-Coated Er To Golgi Transport Vesicle, Cytosol, Endoplasmic Reticulum-Golgi Intermediate Compartment, Mitochondrion, Plasma Membrane, Snare Complex.
Calculated MW:
33kDa
Observed MW:
37kDa
Enables SNAP receptor activity; SNARE binding activity; and protein phosphatase binding activity. Involved in several processes, including autophagosome membrane docking; endoplasmic reticulum to Golgi vesicle-mediated transport; and endoplasmic reticulum-Golgi intermediate compartment organization. Acts upstream of or within protein localization to phagophore assembly site. Located in several cellular components, including autophagosome membrane; endoplasmic reticulum-Golgi intermediate compartment; and mitochondria-associated endoplasmic reticulum membrane. Part of SNARE complex. Colocalizes with HOPS complex.
Purification Method
Affinity purification
Gene ID
55014
RRID
AB_2772449
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse heart, using STX17 Rabbit pAb (CAB17174) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using STX17 Rabbit pAb (CAB17174) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
