The STXBP1 Monoclonal Antibody (CAB9309) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples and has been validated for use in Western blot applications. By binding to the STXBP1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an invaluable resource for studies in neuroscience and neurology.STXBP1, also known as syntaxin-binding protein 1, is crucial for neurotransmitter release and synaptic transmission, playing a pivotal role in neuronal communication and brain function.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
STXBP1 Monoclonal Antibody
SKU:
CAB9309
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1518
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
6812
RRID
AB_2863709
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using STXBP1 Rabbit mAb (CAB9309) at 1:20000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): Mouse skeletal muscle, Rat skeletal muscle. Exposure time: 90 s.
Western blot analysis of lysates from HeLa cells using STXBP1 Rabbit mAb (CAB9309) at 1:5000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using STXBP1 Rabbit mAb (CAB9309) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse pancreas tissue using STXBP1 Rabbit mAb (CAB9309) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
