Description
| Product Name: | Succinate Dehydrogenase (SDH) Activity Assay Kit |
| Product Code: | MAES0229 |
| Sample Type: | Animal Tissue, Cell |
| Assay Time: | 10 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | SDH |
| Detection Instrument: | Microplate reader (590-610 nm, optimum wavelength: 600 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.83 U/L |
| Detection Range: | 0.83-65.42 U/L |
| Inter-assay CV: | 0.04 |
| Intra-assay CV: | 0.02 |
SDH catalyzes the dehydrogenation of succinate to fumarate, with electron transport materials transferring electrons to 2, 6-dichlorophenol indiophenol (DCPIP). Then, the reduced DCPIP is reduced to the oxidized DCPIP, which has a characteristic absorption peak at 600 nm. Therefore, the activity of SDH can be quantified by measure the change OD value at 600 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Normal saline (0.9% NaCl), PBS(0.01 M，pH 7.4). |
| Other Instruments Required: | Centrifuge |
