The SUMO3 Antibody (CAB15724) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. RRID AB_2763138 Gene ID 6612 Swiss Prot Synonym SMT3A; Smt3B; SMT3H1; SUMO-3; SUMO3
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SUMO3 Antibody
SKU:
CAB15724
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SMT3A, Smt3B, SMT3H1, SUMO-3, SUMO3
Positive Sample:
U-87MG, LO2, 293T, Mouse liver, Mouse kidney, Mouse small intestine, Mouse heart, Rat liver, Rat heart
Cellular Localization:
Cytoplasm, Nucleus, Pml Body.
Calculated MW:
12kDa
Observed MW:
17-50kDa
This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. RRID AB_2763138 Gene ID 6612 Swiss Prot Synonym SMT3A; Smt3B; SMT3H1; SUMO-3; SUMO3
Purification Method:
Affinity purification
Gene ID:
6612
RRID:
AB_2763138
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SUMO3 Rabbit pAb (CAB15724) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using SUMO3 Rabbit pAb (CAB15724) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
