The SYNE3 Antibody (CAB13237) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications. It specifically binds to the SYNE3 protein, allowing for its detection and analysis in various cell types.SYNE3 plays a crucial role in maintaining nuclear architecture and cell morphology, making it essential for cellular function.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SYNE3 Antibody
SKU:
CAB13237
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Nucleus Envelope, Nucleus Outer Membrane, Rough Endoplasmic Reticulum, Single-Pass Type Iv Membrane Protein.
Calculated MW:
112kDa
Observed MW:
112kDa
Enables actin filament binding activity and cytoskeleton-nuclear membrane anchor activity. Involved in cytoskeleton organization; establishment of protein localization to membrane; and regulation of cell shape. Located in nuclear membrane. Part of meiotic nuclear membrane microtubule tethering complex. Biomarker of Huntington's disease.
Purification Method
Affinity purification
Gene ID
161176
RRID
AB_2760090
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SYNE3 Rabbit pAb (CAB13237) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using SYNE3 Rabbit pAb (CAB13237) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.