The CD7 Antibody (CAB7650) is a high-quality antibody developed for reliable detection and analysis of target proteins. CD7 is a cell surface glycoprotein that plays a key role in T-cell development and activation. The antibody, produced in rabbits, is highly specific to human CD7 antigen and has been validated for use in applications such as Western blotting.By targeting the CD7 protein, this antibody enables the detection and analysis of CD7 expression in various cell types, making it a valuable asset for immunology research.
This antibody is validated for use in WB, FC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
CD7 Antibody
SKU:
CAB7650
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GP40, TP41, Tp40, LEU-9, CD7
Positive Sample:
Jurkat, U-937, Rat spleen
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
25kDa
Observed MW:
38-40kDa
This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
Purification Method
Affinity purification
Gene ID
924
RRID
AB_2768795
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CD7 Rabbit pAb (CAB7650) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from Rat spleen, using CD7 Rabbit pAb (CAB7650) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.