Based on the information provided in the URL provided, the TAF1A Antibody (PAC052306) is a highly specific research tool designed for investigating the TAF1A protein, a key component of the RNA polymerase II transcription factor complex. This polyclonal antibody is produced using rabbits and has been extensively validated for use in various experimental applications. TAF1A, also known as TATA-box binding protein associated factor 1A, is essential for the proper functioning of RNA polymerase II, which is responsible for transcribing protein-coding genes in eukaryotic cells. By targeting the TAF1A protein, researchers can gain valuable insights into the molecular mechanisms governing gene expression and transcriptional regulation. The TAF1A Antibody is suitable for use in techniques such as Western blotting, immunoprecipitation, and immunofluorescence, allowing for the detection and analysis of TAF1A in different cell types and experimental conditions.
Its high specificity and sensitivity make it an ideal tool for studies in molecular biology, genetics, and biochemistry. Understanding the role of TAF1A in transcriptional regulation is crucial for advancing our knowledge of gene expression dynamics and the development of therapies targeting gene expression processes. By using the TAF1A Antibody in research, scientists can unravel the complex regulatory networks controlling gene activity and potentially uncover new therapeutic strategies for a variety of diseases.
Immunohistochemistry of paraffin-embedded human brain tissue using PACO52306 at dilution of 1:100.
Western Blot. Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, PC-3 whole cell lysate. All lanes: TAF1A antibody at 2.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 40 kDa. Observed band size: 53, 40 kDa.
Background:
Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits.
TAF1A: Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. 2 isoforms of the human protein are produced by alternative splicing.
Biological Process: positive regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; transcription from RNA polymerase I promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter
NCBI Summary:
This gene encodes a subunit of the RNA polymerase I complex, Selectivity Factor I (SLI). The encoded protein is a TATA box-binding protein-associated factor that plays a role in the assembly of the RNA polymerase I preinitiation complex. Alternate splicing results in multiple transcript variants encoding multiple isoforms.[provided by RefSeq, Jan 2011]
