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Product Name:
TC (Total cholesterol) ELISA Kit
Alias:
TC ELISA Kit, Total cholesterol ELISA Kit
SKU:
UNFI0113
Size:
96T
Species:
Universal
Sample Type:
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method:
Competitive ELISA, Coated with Antigen
Detection Wavelength:
OD450
Reaction Duration:
2 hours
Range:
15.625-1000ng/ml
Sensitivity:
9.375ng/ml
Storage:
2-8°C(Sealed), Don't cryopreserve.
Specificity:
Specifically binds with TC, no obvious cross reaction with other analogues.
Component
Size (96T)
Storage
ELISA Microplate (Dismountable)
8×12
Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 months at -20°C
Lyophilized Standard
2 vials
Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 months at -20°C
Biotin-labeled Antibody (Concentrated, 100X)
60 ul
2-8°C (Avoid Direct Light)
HRP-Streptavidin Conjugate (SABC, 100X)
120 ul
TMB Substrate
10 ml
Sample Dilution Buffer
20 ml
2-8°C
Antibody Dilution Buffer
10 ml
SABC Dilution Buffer
10 ml
Stop Solution
10 ml
Wash Buffer (Concentrated, 25X)
30 ml
Plate Sealer
5 pieces
Product Description
1 copy
Other materials and equipment required:
Microplate reader (wavelength: 450nm)
37°C incubator (CO2 incubator for cell culture is not recommended.)
Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips (Calibration is required before use.)
Sterile tubes and Eppendorf tubes with disposable tips
Absorbent paper and loading slot
Deionized or distilled water
Recovery:
Add a certain amount of TC into the sample. Calculate the recovery by comparing the measured value with the expected amount of TC in the sample.
Matrix
Recovery range(%)
Average(%)
serum(n=10)
85-105
97
EDTA plasma(n=10)
94-104
99
Heparin plasma(n=10)
86-105
99
Linearity:
Dilute the sample with a certain amount of TC at 1:2, 1:4 and 1:8 to get the recovery range.
Sample
1:2
1:4
1:8
serum(n=10)
93-104%
89-98%
86-100%
EDTA plasma(n=10)
92-102%
85-101%
85-100%
Heparin plasma(n=10)
85-98%
89-98%
91-100%
Precision(%):
Item
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/ml)
31.23
121
502.3
31.07
124.62
505
Standard deviation
1.55
5.09
22.65
1.36
5.87
22.88
CV(%)
4.96
4.21
4.51
4.39
4.71
4.53
Stability:
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5)
37°C for 1 month
2-8°C for 6 months
Average(%)
80
95-100
Step 1:
Wash the plate twice before adding the standard and sample.
Step 2:
Add 50ul standard or sample into each well. Immediately add 50ul biotin-labeled antibody working solution into each well (The tip can't be reused after touching the liquid.), gently shake the plate for 1min, seal closely and statically incubate for 45 minutes at 37°C.
Washing: Wash the plate three times and immerse for 1min each time.
Step 3:
Add 100ul HRP-Streptavidin Conjugate (SABC) working solution into each well, seal the plate and statically incubate for 30 minutes at 37°C.
Washing: Wash the plate five times and immerse for 1min each time.
Step 4:
Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)
Step 5:
Add 50ul stop solution. Read at 450nm immediately and calculate.
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