The TDO2 Antibody (CAB13182) is a high-quality antibody developed for reliable detection and analysis of target proteins. TDO2 plays a crucial role in the regulation of immune responses, inflammation, and neurotransmission.This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the TDO2 protein, researchers can analyze its expression levels and activity in various cell types, providing insights into its role in disease states such as cancer, autoimmune disorders, and neurological conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TDO2 Antibody
SKU:
CAB13182
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
VLQN MRVP YNRR HYRD NF
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TO, TDO, TPH2, TRPO, HYPTRP, TDO2
Positive Sample:
HT-29, Mouse liver, HuH-7
Cellular Localization:
Cytosol.
Calculated MW:
48kDa
Observed MW:
48kDa
This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism.
Purification Method
Affinity purification
Gene ID
6999
RRID
AB_2760033
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TDO2 Rabbit pAb (CAB13182) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from HuH-7 cells using TDO2 Rabbit pAb (CAB13182) at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
