The TDO2 Antibody (CAB6766) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the TDO2 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an excellent choice for studies in immunology, cancer research, and metabolic disorders.TDO2, also known as tryptophan 2,3-dioxygenase, is a key player in the kynurenine pathway, which influences immune tolerance and response to inflammation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
TDO2 Antibody
SKU:
CAB6766
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TO, TDO, TPH2, TRPO, HYPTRP, TDO2
Positive Sample:
HUH-7
Cellular Localization:
Cytosol.
Calculated MW:
48kDa
Observed MW:
48kDa
This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism.
Purification Method
Affinity purification
Gene ID
6999
RRID
AB_2767349
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HUH-7 cells, using TDO2 Rabbit pAb (CAB6766) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of A-431 cells using TDO2 Rabbit pAb (CAB6766) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
