The TEAD3 Antibody (CAB7454) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot and immunohistochemistry applications.TEAD3 is a key player in the Hippo signaling pathway, which is involved in controlling organ size and tissue regeneration. Dysregulation of TEAD3 has been linked to various diseases, including cancer and cardiovascular disorders.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TEAD3 Antibody
SKU:
CAB7454
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TEF5, TEAD5, TEF-5, DTEF-1, ETFR-1, TEAD-3, TEAD3
Positive Sample:
Mouse heart, Mouse lung, Rat heart
Cellular Localization:
Nucleus.
Calculated MW:
49kDa
Observed MW:
49kDa
This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is predominantly expressed in the placenta and is involved in the transactivation of the chorionic somatomammotropin-B gene enhancer. Translation of this protein is initiated at a non-AUG (AUA) start codon.
Purification Method
Affinity purification
Gene ID
7005
RRID
AB_2767985
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TEAD3 Rabbit pAb (CAB7454) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human placenta using TEAD3 Rabbit pAb (CAB7454) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal immunofluorescence analysis of U-2 OS cells using TEAD3 Rabbit pAb (CAB7454) at dilution of 1:400. Blue: DAPI for nuclear staining.
