The CLEC3B/Tetranectin Monoclonal Antibody (CAB4387) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables calcium ion binding activity; heparin binding activity; and kringle domain binding activity. Involved in bone mineralization and cellular response to transforming growth factor beta stimulus. Located in cytoplasm; extracellular space; and granular component. Part of collagen-containing extracellular matrix. Implicated in osteoarthritis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CLEC3B/Tetranectin Monoclonal Antibody
SKU:
CAB4387
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0993
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TN, TNA, MCDR4, CLEC3B/Tetranectin
Positive Sample:
Human plasma, Mouse lung, Rat lung
Cellular Localization:
Secreted.
Calculated MW:
21kDa
Observed MW:
23-25kDa
Enables calcium ion binding activity; heparin binding activity; and kringle domain binding activity. Involved in bone mineralization and cellular response to transforming growth factor beta stimulus. Located in cytoplasm; extracellular space; and granular component. Part of collagen-containing extracellular matrix. Implicated in osteoarthritis.
Purification Method
Affinity purification
Gene ID
7123
RRID
AB_2863259
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CLEC3B/CLEC3B/Tetranectin Rabbit mAb (CAB4387) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of C6 cells using CLEC3B/CLEC3B/Tetranectin Rabbit mAb (CAB4387) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
