Description
TFEC Antibody (PACO30338)
The TFEC Polyclonal Antibody (PACO30338) is a valuable tool for researchers studying TFEC, a key transcription factor involved in various cellular processes, including autophagy and lysosome biogenesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By targeting the TFEC protein, this antibody allows for accurate detection and analysis in different cell types, making it ideal for investigations in molecular and cellular biology.
TFEC, also known as transcription factor EC, plays a crucial role in the regulation of autophagy and lysosomal function, processes essential for cellular homeostasis and health. Dysregulation of TFEC has been linked to diseases such as neurodegenerative disorders, metabolic syndromes, and cancer. By understanding the function and regulation of TFEC, researchers can gain insights into potential therapeutic targets for these conditions, ultimately leading to the development of novel treatment strategies.
| Antibody Name: | TFEC Antibody (PACO30338) |
| Antibody SKU: | PACO30338 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Transcription factor EC protein (1-197AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot All lanes: TFEC antibody at 3µg/ml + MDA-MB-231 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39, 36, 23, 32 kDa Observed band size: 39 kDa . |
| Background: | Transcriptional regulator that acts as a repressor or an activator. Acts as a transcriptional repressor on minimal promoter containing element F (that includes an E-box sequence). Binds to element F in an E-box sequence-specific manner. Acts as a transcriptional transactivator on the proximal promoter region of the tartrate-resistant acid, phosphatase (TRAP) E-box containing promoter (By similarity). Collaborates with MITF in target gene activation (By similarity). Acts as a transcriptional repressor on minimal promoter containing mu E3 enhancer sequence (By similarity). Binds to mu E3 DNA sequence of the immunoglobulin heavy-chain gene enhancer (By similarity). Binds DNA in a homo- or heterodimeric form. |
| Synonyms: | Transcription factor EC (TFE-C) (Class E basic helix-loop-helix protein 34) (bHLHe34) (Transcription factor EC-like) (hTFEC-L), TFEC, BHLHE34 TCFEC TFECL |
| UniProt Protein Function: | TFEC: Transcriptional regulator that acts as a repressor or an activator. Acts as a transcriptional repressor on minimal promoter containing element F (that includes an E-box sequence). Binds to element F in an E-box sequence-specific manner. Acts as a transcriptional transactivator on the proximal promoter region of the tartrate-resistant acid phosphatase (TRAP) E-box containing promoter. Collaborates with MITF in target gene activation. Acts as a transcriptional repressor on minimal promoter containing mu E3 enhancer sequence. Binds to mu E3 DNA sequence of the immunoglobulin heavy-chain gene enhancer. Binds DNA in a homo- or heterodimeric form. Belongs to the MiT/TFE family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 7q31.2 Cellular Component: nucleoplasm; cytoplasm Molecular Function:protein dimerization activity; DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a member of the micropthalmia (MiT) family of basic helix-loop-helix leucine zipper transcription factors. MiT transcription factors regulate the expression of target genes by binding to E-box recognition sequences as homo- or heterodimers, and play roles in multiple cellular processes including survival, growth and differentiation. The encoded protein is a transcriptional activator of the nonmuscle myosin II heavy chain-A gene, and may also co-regulate target genes in osteoclasts as a heterodimer with micropthalmia-associated transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | O14948 |
| NCBI GenInfo Identifier: | 74705481 |
| NCBI Gene ID: | 22797 |
| NCBI Accession: | O14948.1 |
| UniProt Secondary Accession: | O14948,Q5H9U8, Q709A4, Q8N6J9, B2R8X5, |
| UniProt Related Accession: | O14948 |
| Molecular Weight: | 347 |
| NCBI Full Name: | Transcription factor EC |
| NCBI Synonym Full Names: | transcription factor EC |
| NCBI Official Symbol: | TFECÂ Â |
| NCBI Official Synonym Symbols: | TCFEC; TFE-C; TFECL; TFEC-L; bHLHe34; hTFEC-LÂ Â |
| NCBI Protein Information: | transcription factor EC; class E basic helix-loop-helix protein 34 |
| UniProt Protein Name: | Transcription factor EC |
| UniProt Synonym Protein Names: | Class E basic helix-loop-helix protein 34; bHLHe34; Transcription factor EC-like; hTFEC-L |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | TFECÂ Â |
| UniProt Entry Name: | TFEC_HUMAN |
| Antibodies |
| TFEC Antibody (PACO12716) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
