The TFR2 Antibody (CAB9845) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the TFR2 protein, this antibody allows for detection and analysis in a variety of cell types, making it ideal for investigations in the fields of biochemistry and cell biology.TFR2 plays a crucial role in iron metabolism, including the regulation of iron uptake and storage within cells.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
TFR2 Antibody
SKU:
CAB9845
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HFE3, TFRC2, TFR2
Positive Sample:
K-562, Mouse liver
Cellular Localization:
Cell Membrane, Cytoplasm, Single-Pass Type Ii Membrane Protein.
Calculated MW:
89kDa
Observed MW:
100kDa
This gene encodes a single-pass type II membrane protein, which is a member of the transferrin receptor-like family. This protein mediates cellular uptake of transferrin-bound iron, and may be involved in iron metabolism, hepatocyte function and erythrocyte differentiation. Mutations in this gene have been associated with hereditary hemochromatosis type III. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Purification Method
Affinity purification
Gene ID
7036
RRID
AB_2772579
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using TFR2 Rabbit pAb (CAB9845) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using TFR2 Rabbit pAb (CAB9845) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
