The TFRC Antibody (CAB5865) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated through immunization of rabbits, exhibits high specificity and sensitivity towards TFRC in human samples and is validated for use in Western blot assays.TFRC is crucial for maintaining iron homeostasis in cells and is commonly dysregulated in various diseases, including cancer, anemia, and neurodegenerative disorders. By targeting TFRC with this polyclonal antibody, researchers can accurately detect and quantify TFRC levels in different cell types, allowing for detailed investigations into the role of iron metabolism in health and disease.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TFRC Antibody
SKU:
CAB5865
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Membrane, Melanosome, Secreted, Single-Pass Type Ii Membrane Protein.
Calculated MW:
85kDa
Observed MW:
100kDa
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.
Purification Method
Affinity purification
Gene ID
7037
RRID
AB_2766615
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells using CD71/Transferrin Receptor Rabbit pAb (CAB5865) at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD71/Transferrin Receptor Rabbit pAb (CAB5865) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta using CD71/Transferrin Receptor Rabbit pAb (CAB5865) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
