Thioredoxin system, as one of the most important antioxidant systems in the body, can stabilize the redox balance of organisms, regulate signal transduction. It is also related to cell REDOX reaction, nucleic acid metabolism, cell growth and tumorigenesis. Thioredoxin reductase (TrxR) is a NADPH-dependent dimer selenase containing the flavin adenine dinucleotide (FAD) domain and is a member of the pyridine nucleotide-disulfide oxidoreductase family. The thioredoxin system consists of NADPH, thioredoxin reductase and thioredoxin. The TrxR catalytic substrate makes the reducing agent reduce the color developer with a characteristic absorption peak at 412 nm.
