The TIMP4 Antibody (CAB6416) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting TIMP4 in human samples, making it ideal for use in applications such as Western blotting.TIMP4 is known to play a crucial role in various physiological processes, including tissue development, wound healing, and cardiovascular health. Dysregulation of TIMP4 expression has been linked to diseases such as cancer, cardiovascular disorders, and fibrosis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TIMP4 Antibody
SKU:
CAB6416
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TIMP-4, TIMP4
Positive Sample:
HeLa, U-87 MG, A549, Mouse heart, Mouse ovary, Rat brain
Cellular Localization:
Secreted.
Calculated MW:
26kDa
Observed MW:
25kDa
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.
Purification Method
Affinity purification
Gene ID
7079
RRID
AB_2767018
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using TIMP4 Rabbit pAb (CAB6416) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunofluorescence analysis of A549 cells using TIMP4 Rabbit pAb (CAB6416) at a dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
