The TLE1 Antibody (CAB13360) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for use in Western blot applications.TLE1 is a key player in the regulation of gene expression and has been implicated in numerous diseases, including cancer. By targeting TLE1, researchers can gain insights into the mechanisms of cancer development and progression, potentially leading to the development of novel therapeutic strategies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
TLE1 Antibody
SKU:
CAB13360
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ESG, ESG1, GRG1, TLE-1, TLE1
Positive Sample:
Mouse liver
Cellular Localization:
Nucleus.
Calculated MW:
83kDa
Observed MW:
83kDa
Enables identical protein binding activity and transcription corepressor activity. Involved in negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of anoikis; and regulation of gene expression. Located in cytosol and nucleoplasm. Part of beta-catenin-TCF complex.
Purification Method
Affinity purification
Gene ID
7088
RRID
AB_2760218
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse liver, using TLE1 Rabbit pAb (CAB13360) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
