The TLK1 Polyclonal Antibody (CAB21987) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By targeting the TLK1 protein, this antibody enables precise detection and analysis in various cell types, making it an excellent choice for studies in molecular biology and cancer research.TLK1, also known as Tousled-like kinase 1, plays a crucial role in maintaining genomic stability and proper cell division.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TLK1 Polyclonal Antibody
SKU:
CAB21987
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PKU-beta, TLK1
Positive Sample:
HeLa, Jurkat, NIH/3T3
Cellular Localization:
Nucleus.
Calculated MW:
87kDa
Observed MW:
87kDa
The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
9874
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TLK1 Rabbit pAb (CAB21987) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
