The TLR5 Monoclonal Antibody (CAB8765) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting TLR5 in various samples, particularly human tissues. Validated for use in Western blot applications, it effectively binds to the TLR5 protein, enabling precise detection and analysis in a variety of cell types.TLR5 is a crucial component of the innate immune system, recognizing and responding to bacterial pathogens through the activation of inflammatory pathways. Studies have shown that dysregulation of TLR5 can contribute to the development of inflammatory diseases and even cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TLR5 Monoclonal Antibody
SKU:
CAB8765
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1294
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SLE1, TIL3, SLEB1, MELIOS, TLR5
Positive Sample:
HeLa, A549
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
98kDa
Observed MW:
105kDa
This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.
Purification Method
Affinity purification
Gene ID
7100
RRID
AB_2863604
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TLR5 Rabbit mAb (CAB8765) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
